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. 2008 Dec 11;3(12):e3910. doi: 10.1371/journal.pone.0003910

Figure 6. Age-dependant down-regulation of SRF expression in skeletal muscle of mice and human.

Figure 6

(A) Immunoblot analysis of protein extracts from 2, 10 and 15 month-old control muscles using anti-SRF antibody. Anti-α-Tubulin is used as a loading control. (B) Quantitative real-time PCR was performed on RNAs prepared from Gastrocnemius muscles of control mice 2 (white) and 15 (grey) months old. Mean expression levels for SRF, Skeletal and Cardiac α-actin mRNAs was normalized using Cyclophilin transcript as reference. (n = 4). *P<0.05 over control 2 month-old. (C) Immunoblot analysis of protein extracts from human skeletal muscle (24, 55, 80 and 83 years old) using anti-SRF antibody. Ponceau staining is used as a loading control.