Fig. 1.

Confocal microscopy analysis of the nuclear lamina in polyQ-expanded huntingtin-expressing cells. CHO cells were transfected with huntingtin exon 1 with 103 glutamines tagged with EGFP (Htt103Q). Cells were stained with an antibody to lamin B1 and imaged by laser scanning confocal microscopy. Perinuclear Htt103Q aggregates are associated with a distortion of the nuclear envelope, clearly evident with lamin B1 staining. By contrast, cells with cytoplasmic aggregates (arrows) have an intact nuclear envelope. (A) EGFP fluorescence, (B) lamin B1 staining, and (C) merged signals. Scale bar = 10 μm.