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. Author manuscript; available in PMC: 2008 Dec 4.
Published in final edited form as: Biochemistry. 2004 Aug 10;43(31):10285–10294. doi: 10.1021/bi0495432

Figure 8.

Figure 8

Changes in Trp fluorescence of GLTP induced by glycolipid liposomes. Spectra were recorded as described in the Figure 2 caption. Spectral subtraction of liposome blanks was routinely used. Corrections for liposome-induced reduction of Trp excitation and emission were estimated from measurements of Trp plus liposomes. Controls that maximally suppress liposome-scattering artifacts using a cross-oriented configuration of the polarizers (Expol = 90° and Empol = 0°) did not significantly change the outcome (34). (A) Porcine brain GalCer/POPC (1:4); (B) 18:1 GalCer/POPC (1:4); (C) POPC; (D) egg sphingomyelin.