The data are presented as the change in fluorescence intensity (Fo - F) plotted as a function of ligand concentration. PPARαΔAB (0.1μM) was titrated with 0-2500nM of the following ligands: panel A, behenic acid (C22:0); panel B, docosapentaenoic acid (C22:5); panel C, docosahexaenoic acid (C22:6); panel D, behenoyl-CoA (C22:0-CoA); panel E, docosapentaenoyl-CoA (C22:5-CoA); panel F, docosahexaenoyl-CoA (C22:6-CoA). Values represent the mean ± SE, n=4-5. Insets, linear plot of the binding curve from each panel.