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. Author manuscript; available in PMC: 2008 Dec 4.
Published in final edited form as: Biochemistry. 2006 Jun 20;45(24):7669–7681. doi: 10.1021/bi060198l

FIG. 4. Interaction of branched-chain fatty acids and fatty acyl-CoAs with PPARαΔAB: Direct binding based on quenching of PPARαΔAB aromatic amino acid fluorescence emission.

FIG. 4

The data are presented as the change in fluorescence intensity (Fo - F) plotted as a function of ligand concentration. PPARαΔAB (0.1μM) was titrated with 0-2500nM of the following ligands: panel A, phytanic acid (C16:0-branched); panel B, pristanic acid (C16:0-branched); panel C, bromopalmitate (C16:0-Br); panel D, phytanoyl-CoA (C16:0-CoA); panel E, pristanoyl-CoA (C16:0-CoA); panel F, S-hexadecyl-CoA (C16:0-S-CoA). Values represent the mean + SE, n=4-5. Insets, linear plot of the binding curve from each panel.