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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: J Immunol. 2008 Oct 1;181(7):4840–4851. doi: 10.4049/jimmunol.181.7.4840

Figure 2. Schematic and expression of ADAP mutant constructs.

Figure 2

(A) Scale diagram of ADAP expression constructs used in this study. Amino acid numbering is given for the murine ADAP p130 isoform. Abbreviations: PRO, proline-rich domain; E/K, glutamic acid and lysine-rich domain; hSH3, N-terminal (N) or C-terminal (C) helical SH3 domain; EVH1, Ena/Vasp homology domain. Asterisks (*) are used to denote the position of tyrosines 547, 549, 584, 615, 687 found in phosphorylation consensus motifs. (B) Freshly harvested naïve DO11.10/hCAR/ADAP+/+ (WT) or ADAP-/- (KO) lymphocytes were transduced with adenoviruses encoding the indicated constructs or Thy1.1 control adenovirus (ctrl) and fixed and stained with an anti-Thy1.1 antibody and either anti-ADAP antibody or non-immune sheep serum (IgG) and analyzed by flow cytometry. (C) Same as in (B) except the indicated constructs were stained with an anti-hemaglutinin (HA) antibody. Expression profiles are representative of at least three independent analyses performed for each construct shown.