Table 1.
Quantification of amount of MSY2 present in insoluble fraction following either KCl or RNase treatment
| Treatment | ||||||
|---|---|---|---|---|---|---|
| KCl [M] | RNase [ng/µl] | |||||
| 0.2 | 0.4 | 0.6 | 1 | 10 | 100 | |
| GV | 80±6 | 51±10 | 17±3 | 84±6 | 60±10 | 10±1 | 
| MII | 46±10 | 33±8 | 4±1 | 43±8 | 17±2 | 3±1 | 
The data are relative to extracted but untreated oocytes or MII eggs and are expressed as mean±SEM (n=3).
Note that extracted but untreated cells are exposed to 0.1 M KCl, which is present in the extraction buffer.