Figure 4. Time-dependent changes in morphology induced by DCVC.
hPT cells were cultured for 5 days in supplemented, serum-free, hormonally-defined DMEM:F12 medium on collagen-coated, 35-mm polystyrene dishes until they were confluent. Cells were then incubated for the indicated times up to 24 hr with either medium (= Control) or 50 μM DCVC. At the indicated times, photomicrographs were taken at 196X magnification on a Carl-Zeiss Confocal Laser Microscope. Bar = 5 μm.