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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: Biochem Pharmacol. 2008 May 28;76(4):552–567. doi: 10.1016/j.bcp.2008.05.016

Figure 6. Time- and concentration-dependent inhibition of cellular respiration by DCVC.

Figure 6

Confluent hPT cells grown on collagen-coated, T-25 flasks were incubated up to 48 hr with PBS (= Control, Con), the indicated concentrations of DCVC, or 1 μM staurosporine (Sts). At the indicated times, cells were released from flasks by brief treatment with trypsin/EDTA, resuspended in PBS at a cell concentration of 2 × 106/ml, and were added to the Oxygraph electrode chamber for measurement of succinate-stimulated O2 consumption. Results are means ± SEM of measurements from 4 separate cell cultures, each deriving from a different kidney, except for incubation with Sts, which were averages of measurements from 2 separate cell cultures. *Significantly different (P < 0.05) from corresponding control values.