Table 1.

Effect of DCVC and mitochondrial inhibitors on ATP concentrations of hPT cells

Incubation	ATP (% Control)
Control	100 ± 5.1
1 mM KCN + 0.1 mM Iodoacetate (KCN/IAA) [0.5 hr]	22.6 ± 7.3
1 μM Antimycin A (AA)	78.7 ± 3.3
1 μM Staurosporine (Sts)	78.7 ± 8.4
50 μM tert-Butyl hydroperoxide (tBH)	18.2 ± 2.1
DCVC:
10 μM	80.0 ± 2.1
50 μM	71.7 ± 3.9
100 μM	60.7 ± 13.4
200 μM	61.0 ± 8.0
300 μM	48.3 ± 8.4

Primary cultures of hPT cells were grown to ∼80% confluence and then incubated with the indicated additions for 24 hr, except for KCN/IAA as noted. Cellular contents of ATP were determined by a luminescence assay and are expressed as the percent of control. Control = 8.63 nmol ATP/mg protein. Results are means ± SEM of measurements from 3 separate cell cultures, each derived from a different kidney. All values were significant different (P < 0.05) from those for Control cells.