. Author manuscript; available in PMC: 2009 Aug 15.

Published in final edited form as: Biochem Pharmacol. 2008 May 28;76(4):552–567. doi: 10.1016/j.bcp.2008.05.016

Table 2.

Effect of DCVC and mitochondrial inhibitors on caspase activities of hPT cells

Incubation	Caspase-3/7		Caspase-8
Incubation
	4 hr	24 hr	4 hr	24 hr
Control	100 ± 6.4	100 ± 4.1	100 ± 5.5	100 ± 4.2
1 μM Antimycin A (AA)	90.5 ± 6.5	115 ± 10	74.6 ± 4.1^*	155 ± 7^*
1 μM Staurosporine (Sts)	212 ± 11^*	262 ± 15^*	175 ± 8^*	137 ± 6^*
DCVC:
10 μM	115 ± 5^*	92.5 ± 4.3	117 ± 6^*	108 ± 4
50 μM	141 ± 8^*	94.1 ± 5.5	107 ± 6	110 ± 4^*
100 μM	105 ± 6	82.3 ± 4.4^*	94.2 ± 4.1	124 ± 10^*
200 μM	85.5 ± 3.5^*	67.7 ± 4.1^*	82.2 ± 4.5^*	97.6 ± 4.4
300 μM	74.7 ± 3.5^*	52.1 ± 3.2^*	72.5 ± 2.7^*	86.4 ± 4.2^*

Primary cultures of hPT cells were grown to ∼80% confluence and then incubated with the indicated additions for 4 hr or 24 hr. At the end of the incubation time, cells were washed,scraped into 100 μl of PBS in 96-well plates, and then 100 μl of either the Caspase-Glo 3/7 or 8 reagent was added. After 2 hr incubation in the dark, plates were read in luminescence mode in a SpectraMax 2 plate reader (Molecular Devices). Results are expressed as relative luminescence normalized to protein relative to controls (set at 100 for each experiment) and are means ± SEM of measurements from 3 separate cell cultures, each derived from a different kidney.

Significantly different (P < 0.05) from the corresponding control value.