Fig. 7.

Dependency of agonist-stimulated CREB phosphorylation on PKA or PI3-kinase activities in SH-SY5Y cells. Effects of the PKA inhibitor H89 (10 μM; 30 min pre-treatment) or the PI3-kinase inhibitor wortmannin (100 nM; 30 min pre-treatment) on CREB phosphorylation stimulated by MCh (1 μM; 1 and 5 min) or bradykinin (300 nM; 1 and 5 min). Cells were also stimulated with forskolin (10 μM)/IBMX (500 μM) for 10 min (Fk). Representative immunoblots for phospho-CREB and total CREB are shown beneath each histogram. Data, normalized to the forskolin/IBMX response (=100%) in the absence of respective inhibitor treatment, are shown as means ± S.E.M. for at least 3 independent experiments. Statistically significant changes in the forskolin/IBMX response caused by H89 treatment are indicated as ^*p < 0.05, ^**p < 0.01.