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The L106P domain was PCR amplified and inserted into YFP-L106P to obtain YFP fusion proteins with one (1x L106P), two (2x L106P) or three (3x L106P) copies of L106P at the C-terminus of YFP. NIH3T3 cells stably expressing these fusion proteins were mock-treated (-) or treated with 1 μM Shield-1 (+) for 24h. Fluorescence of the fusions was determined by flow cytometry.
