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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Dev Biol. 2008 Jun 13;321(1):111–122. doi: 10.1016/j.ydbio.2008.06.002

Figure 3. Constitutive expression of Notch signaling alters lens growth and differentiation.

Figure 3

(A–D) Histological sections from the P0-3.9-GFPCre;Rosa26Notch1IC eyes show a thickening and multilayering of the AEL (indicated by brackets), as well as loss of a definitive cornea. At E18.5 (C,D), radially-aligned nuclei are observed in the P0-3.9-GFPCre;Rosa26Notch1IC lens (arrows in D). ael–anterior epithelial layer, c–cornea, fc–presumptive fiber cells. (E,F) Foxe3, (G,H) E-Cadherin, and (I,J) Pax6 expression inappropriately persist in E12.5 posterior P0-3.9-GFPCre;Rosa26Notch1IC lenses, indicating delayed primary fiber cell differentiation, accompanied by a (K,L) highly reduced γ-crystallin domain. (G,H) p57Kip2 initiates normally in the P0-3.9-GFPCre;Rosa26Notch1IC posterior lens in E-Cadherin-positive cells. Bar in A,C,E = 100 microns, anterior is up in A–L.