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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Dev Biol. 2008 Jun 13;321(1):111–122. doi: 10.1016/j.ydbio.2008.06.002

Figure 4. Constitutive expression of Notch signaling alters expression of lens regulatory proteins.

Figure 4

(A,B) Lens epithelial cells expressing Foxe3 or (C,D) E-Cadherin are inappropriately expanded posteriorly in E14.5 and E18.5 P0-3.9-GFPCre;Rosa26Notch1IC lenses. Arrowheads denote the boundary between the AEL and fiber cells at E14.5. (E,F) Pax6 expression persists in E14.5 or E18.5 P0-3.9-GFPCre;Rosa26Notch1IC posterior lenses. (G,H) Prox1 is highly expressed in E14.5 and E18.5 P0-3.9-GFPCre;Rosa26Notch1IC posterior lenses. (I,K) GFP fluorescence or (J,L) Cre protein directed from P0-3.9-GFPCre is expressed throughout the AEL and posterior lens of E14.5 and E18.5 P0-3.9-GFPCre;Rosa26Notch1IC lenses. Some GFP expression may be directed from the Notch1IC allele, while Cre is only directed from P0-3.9-GFPCre. Note that sections in I and J are from different lenses, while K and L are colabelings of the same section. Bar in A = 100 microns; anterior is up in A–L.