Table 1.
| Antigen/Species | Lab Code | Commercial Source/Code | Reference for IHC10 | Dilution | Donor Species |
|---|---|---|---|---|---|
| 1VAChT (human) | 80153 | Phoenix/ H-V005(2/95bleed) | Schäfer et al. 1995 | 1:600 | rabbit polyclonal |
| 2VAChT (common) | 1624 | Phoenix/ H-V007(bleed 12/99) | this report | 1:600 | goat polyclonal |
| 3VAChT (rat, mouse) | 80259 | Phoenix/ H-V006(2/95bleed) | Weihe et al. 1996 | 1:300 | rabbit polyclonal |
| 4VMAT2 (rat, human) | 80182 | Phoenix/ H-V003(finalbleed) | Erickson et al. 1996 | 1.200 | rabbit polyclonal |
| 5VMAT2 (rat) | 80214 | Phoenix/ H-V004(bleed 2/95) | Weihe et al 1995 | 1.200 | rabbit polyclonal |
| 6VMAT2 (mouse) | W1-2 | Phoenix/ H-V008(finalbleed) | this report | 1:200 | rabbit polyclonal |
| 7TH (primate, rat, mouse) | Chemicon/ AB1542 | Anlauf et al. 2003 | 1:80 | sheep polyclonal | |
| 8DßH (primate, rat, mouse) | Protos/ CA-301 bDBHrab | this report | 1:200 | sheep polyclonal | |
| 9AADC (primate, rat, mouse) | Chemicon/ AB1569 | this report | 1:200 | rabbit polyclonal |
1-6, Antigen epitope sequence (includes cysteine at N-terminus added when necessary for conjugation to KLH (keyhole limpet hemocyanin) through maleimide):
VAChT (human): CEDDYNYYYTRS
VAChT (common): CTRSRSERDVLLDEPPQGLYDAVRLRE
VAChT (rat, mouse): CEDDYNYYSRS
VMAT2 (rat, human): CTQNNIQSYPIGEDEESESD
VMAT2 (rat): CTQNNVQSYPIGDDEESESD
VMAT2 (mouse): CTQNNVQPYPVGDDEESESD
TH (native tyrosine hydroxylase purified from rat pheochromocytoma)
DßH (native dopamine ß-hydroxylase purified from bovine adrenal medulla)
AADC (recombinant bovine dopa decarboxylase expressed in E. coli and purified from inclusion bodies)
References for use of these antibodies immunohistochemistry (IHC) are to Materials and Methods section of this paper, or to previous reports, describing in detail the methods for use of these antibodies in immunohistochemical detection of antigen in mammalian tissues. For antibodies against TH, DßH, and AADC, the product sheets of the individual suppliers (TH and AADC, lots 24080597 and 23050121 respectively, Chemicon, Temecula, CA; DBH, Protos Biotech, New York; all other antibodies Phoenix Pharmaceuticals, Belmont CA). ‘Final bleed’ is the agreed-upon designation for the single existing bleed used by Weihe and Eiden laboratories and commercially available through Phoenix Pharmaceuticals. This applies to 80182, and W1-2.
Abbreviations: VAChT, vesicular acetycholine transporter; VMAT2, vesicular monoamine transporter type 2; TH, Tyrosine hydroxylase; AADC, aromatic amino acid decarboxylase also referred to as DDC, dopa-decarboxylase; DßH, dopamine ß-hydroxylase.