Figure 5.

p19^Arf is elevated in BubR1 hypomorphic tissues with high p16^Ink4a. (a) Skeletal muscles of wild-type and BubR1^H/H mice of various ages were analysed for p19^Arf expression by qRT–PCR. All values were normalized to GAPDH. Data are mean ± s.d. (n = 3 mice were used per genotype and age group). (b) Relative expression of p19^Arf in various tissues of 2-month-old BubR1^H/H and BubR1^+/+ mice as measured by qRT–PCR. Data are mean ± s.d. (n = 3 males per genotype). All values were normalized to GAPDH. Relative expression is to wild-type samples. (c) Western blots of eye and fat extracts from 2-month-old BubR1^+/+ and BubR1^H/H mice probed with anti-p19^Arf and p15^Ink4b antibodies. Anti-tubulin antibody was used as a loading control. Uncropped images of the scans are shown in Supplementary Information, Fig. S6b, c. (d) Relative expression of p19^Arf in skeletal muscle (gastrocnemius), subdermal adipose, fat deposits and eyes of 2-month-old wild-type, BubR1^H/H and BubR1^H/H;p16^Ink4a−/− mice as determined by qRT–PCR. Data are mean ± s.d. (n = 3 males per genotype). All values were normalized to GAPDH. Relative expression is to wild-type samples.