Table 2.

Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain BJ5464+pRS314-β were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.

	PDI level during expression in BJ strain (relative to wt)	PDI level during expression in hPDI strain (relative to wt)	Intracellular β-glucosidase in BJ strain (relative to wt)	Intracellular β-glucosidase in hPDI strain (relative to wt)
wt β-glucosidase	1	12-15	1	0.8-1.2
C75S β-glucosidase	1-1.3	12-15	0.8-1.2	0.8-1.2