Skip to main content
. Author manuscript; available in PMC: 2008 Dec 4.
Published in final edited form as: J Am Chem Soc. 2006 Nov 15;128(45):14476–14477. doi: 10.1021/ja066360p

Fig 2.

Fig 2

Fluorescence assays for GPR30 binding and activation with 2 and 3. a) GPR30 competitive binding of E2, 17α-E2, 2, and 3 with fluorescent estradiol derivative E2Alexa using permeabilized COS7 cells. b) Calcium mobilization assay for GPR30 and ERα in transfected COS7 cells vs concentration of E2, and complexes 2 and 3 (each at 10, 100, and 1000 nM).