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. 2008 Nov 3;1:5. doi: 10.1186/1756-8935-1-5

Figure 2.

Figure 2

Engineered subtelomeric markers. A-C) Top of each panel shows a cartoon map (not to scale) of inserted selectable markers at telomeres VR (contig 7.37, panel A), VIIL (contig 7.251, panel B) and IIR (contig 7.77, panel C). Vertical striped bars represent telomeric (TTAGGG)n repeats and diagonal striped bars in A represent Pogo LTRs [29]. Gray triangles on Tel VIIL (B) represent CenVII repeat element (194–280 bp from end) and a second unnamed repeat element (280–1077 bp from end). Southern blots of DNA from strains 1 (N150; WT), 2 (N3120; Tel VR::hph), 3 (N3440; TelVIIL::bar), 4 (N3456; TelIIR::bar) digested with the enzyme named at the top of each autoradiogram. Probes used are indicated under each panel and shown as gray bars in the maps.