Table 2.
Genetic variation in the intron containing Mal d 1 genes of linkage group 16 among ten apple cultivars.
| Iso-allergen | Varianta | Sil. mut | gDNA #b | Cultivarc | GenBank Accns | |||||||||
| GD | PS | IM | CO | JO | RD | FJ | DS | PM | FS | |||||
| 1.02 | 01 | 1 | 1 | # | # | # | # | # * | AY789240–AY789241, AY827646–AY827648 | |||||
| 01 | 2 | 2 | * | AY789239 | ||||||||||
| 01 | 3 | 3 | # * | * | * | * | * | * | AY827649–AY827654 | |||||
| 01 | 4 | 4 | * | # | AY827655–AY827656 | |||||||||
| 01 | 5 | 5 | # | AY827657 | ||||||||||
| 01 | 6 | 6 | # | AY827658 | ||||||||||
| 01 | 7 | 7 | + | AY827659 | ||||||||||
| 09 | 1 | 8 | + | AY827660 | ||||||||||
| 1.04 | 04 | 1 | 1 | * | AY789242 | |||||||||
| 04 | 2 | 2 | # e | # | # | # | # | # * | AY789243–AY789244, AY827661–AY827664 | |||||
| 05 | 1 | 3 | + | AY827665 | ||||||||||
| 06 | 1 | 4 | # | AY827666 | ||||||||||
| 07 | 1 | 5 | + | AY827667 | ||||||||||
| ps1 | 1 | 6 | # * | * | * | * | AY827668–AY827671 | |||||||
| ps2 | 1 | 7 | * | * | * | # | AY827672–AY827675 | |||||||
| 1.06A | 01 | 1 | 1–10 | # | # | # | # * | AY789249, AY827689–AY827691 | ||||||
| 01 | 1 | 2–11 | # | AY789250 | ||||||||||
| 02 | 1 | 3–16 | * | AY789248 | ||||||||||
| 02 | 2 | 4–6 | # * | * | * | * | * | * | AY827692–AY827697 | |||||
| 02 | 3 | 5–10 | # | # | AY827698–AY827699 | |||||||||
| 02 | 4 | 6–7 | + | AY827700 | ||||||||||
| 03 | 1 | 7–7 | * | # | + | AY827701–AY827703 | ||||||||
| 1.06B | 01 | 1 | 1 | # | # | # | # | # * | AY789251, AY827704–AY827707 | |||||
| 02 | 1 | 2 | * | * | * | * | # * | # | AY789252, AY827708–AY827712 | |||||
| 03 | 1 | 3 | # | * | AY789253, AY827713 | |||||||||
| 03 | 2 | 4 | # | AY827714 | ||||||||||
| 04 | 1 | 5 | * | * | AY827715–AY827716 | |||||||||
| 05 | 1 | 6 | + | AY827717 | ||||||||||
| 05 | 2 | 7 | + | AY827718 | ||||||||||
| 1.06C | 01 | 1 | 1 | # | # | # | # | # * | AY789254, AY827719–AY827722 | |||||
| 02g | 1 | 2 | * | AY789255 | ||||||||||
| 03g | 1 | 3 | * | * | * | * | # * | + | # | AY789256, AY827723–AY827725 | ||||
| 04 | 1 | 4 | # | + | AY827726 | |||||||||
| 05 | 1 | 5 | # | AY827727 | ||||||||||
| 06 | 1 | 6 | * | * | AY827728 | |||||||||
aVariant numbers of are according to the allergen list (see Table 1), those for Mal d 1.05 and Mal d 1.06 are according to Gao et al. [15].
b, c, d as in Table 1
e # and * For LG16, alleles that have the same symbol are in coupling phase with each other. This information was obtained by their co-segregation patterns in mapping progenies (Prima × Fiesta and Jonathan × Prima [15], and over pedigrees (Fiesta = Cox × Idared [= Jonathan × Wagner Apfel]); Ingrid Marie = Cox × open pollinated and Fuji = Ralls Janet × Delicious; Red Delicious is a colour mutant of Delicious.
fps1 and ps2 of Mal d 1.04 refer to pseudo-alleles.
g The allele specific markers for these alleles were derived from Table 3 of Gao et al. 2005 [15] whereby we took into account that their marker for Mal d 1.06C02 actually amplifies Mal d 1.06C03 and visa versa, thus harmonizing an inconsistency between their tables 2 and 3 in the assignment of allele and marker names to the AY789255 and AY789255 sequences.