Table 5.

Evaluation of in silico (design) probes against hybridization results with R^U = 1.0 and R^L = 0.5 for five categories of probes

Category (targets)	In silico	Experimental
		Class A	Class B	Class C
I (B. mallei ATCC 23344)	0	0 (0%)	0 (0%)	0 (0%)
II (B. mallei NCTC 10229)	2	0 (0%)	2 (100%)	0 (0%)
III (B. pseudomallei K96243)	523	420 (80%)	53 (10%)	50 (10%)
IV (B. mallei ATCC 23344 and B. mallei NCTC 10229)	21	17 (81%)	2 (10%)	2 (10%)
V (B. mallei ATCC 23344, B. mallei NCTC 10229 and B. pseudomallei K96243)	431	184 (43%)	12 (3%)	236 (55%)

Probes that behave as expected are categorized as Class A; i.e., these probes have normalized hybridization intensity greater than R^U with intended targets and less than R^L with non-targets. Class B probes have normalized hybridization intensity greater than R^L with non-targets, and Class C probes have normalized hybridization intensity less than R^U with the intended targets.