Figure 3.

Gαq-family immunoreactivity in different taste fields. Inverted grayscale images of single confocal image plane of immunofluorescence showing Gαq/11/14-immunoreactivity in different taste fields. Acquisition and display parameters are matched to permit comparison of fluorescence intensity between image sets (A-C), (D-E) and (F-G). The heavy staining is largely membrane-associated and outlines the entire cell. Vallate (A & F) and foliate field (D) taste buds each contain several Gαq/11/14-immunoreactive taste cells. Rare immunoreactive taste cells occur in palatal taste buds (B) but essentially no Gq/11/14immunoreactivity is detected in fungiform field taste buds (C & E). Similarly, pharyngeal taste buds exhibit little or no specific immunofluorescence. Scale bar equals 10 μm for panels A-C and 25 μm in D-G.