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. 2008 Nov 13;9:110. doi: 10.1186/1471-2202-9-110

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Copyright © 2008 Tizzano et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gq/11/14 immunoreactivity in Type II cells. (A) Co-localization of Gq/11/14 (red), Gαgus (blue) in the vallate papilla of a TrpM5-GFP (green) animal. TrpM5 is a marker of Type II taste cells. Gq/11/14 stains about half (14 of 27) of the TrpM5-GFP cells. Usually the Gαq-family immunoreactive cells are different from those positive for Gαgus (blue). A', A", represent single staining of TrpM5-GFP TBs used to obtain the merged pictures A. The figure in Additional file 1 shows single plane confocal images from this image set for comparison. (B) PGP9.5 (blue), a marker of Type III cells, does not co-localize with either Gq/11/14 (red) or TrpM5 (green). The figure in Additional file 2 shows single plane confocal images from this image set for comparison. Scale bar 20 μM.