Table 1.
T1R & G-protein co-localization in Taste Buds
| Immunocytochem. | Single Cell RT-PCR | ||||
| TrpM5 with | % | TrpM5 with | % | ||
| Gq/11/14 | 14/27 | 51.9 | Gα14 | 13/21 | 61.9 |
| Gαgus | 17/27 | 63.0 | Gαgus | 15/21 | 71.4 |
| Gq/11/14 + Gαgus | 4/27 | 14.8 | Gα14 + Gαgus | 7/21 | 33.3 |
| Gq/11/14 with | Gα14 with | ||||
| T1R3(GFP) | 30/35 | 85.7 | T1R3 | 13/14 | 92.9 |
| T1R2 | 10/14 | 71.4 | |||
| T1R3 + T1R2 | 10/14 | 71.4 | |||
| T1R3(GFP) | T1R3+T1R2 with | ||||
| (includes T1R1 & TT1R2) | Gαq | 5/10 | 50.0 | ||
| Gq/11/14 | 30/41 | 73.1 | Gα14 | 10/10 | 100.0 |
| Gαgus | 11/41 | 26.8 | Gαgus | 4/10 | 40.0 |
Results from immunocytochemistry are not significantly different (p > 0.05) from data from single cell RT-PCR (Fisher's Exact Test). The immunocytochemical data and RT-PCR data showing co-expression of gustducin with T1Rs is not directly comparable since some cells express only T1R3 and not T1R2+T1R3