Skip to main content
. 2008 Nov 19;105(48):18924–18929. doi: 10.1073/pnas.0810230105

Fig. 1.

Fig. 1.

Effect of an NO donor, sGC activators, and a NOS inhibitor on EZ1 (murine ES) cells. The EZ1 cells were differentiated and exposed to NOC-18 (1 μM), BAY 41-2272, YC-1 (3 μM), l- NAME (1 mM), and a combination of NOC + BAY 41-2272. or NOC + YC-1 on days 0, 5, and 7 and harvested on day 10. (A and C) mRNA levels for the indicated genes were analyzed by real-time PCR and normalized by using the housekeeping gene GAPDH and presented as fold expression compared with day 0. Day 0 represents undifferentiated cell culture usually collected before subjecting cells to differentiation. The data were analyzed by using the 2−ΔΔCt method. Error bars indicate ± SEM. Significance using paired Student's t test is indicated: *, P < 0.05; **, P < 0.01. n = 3. (B) The protein from EZ1 cells untreated or treated with NOC-18 (1 μM), BAY 41-2272, YC-1 (3 μM), l- NAME (1 mM), or the combination exposed on days 0, 5, and 7 and harvested on day 10 was detected with specific antibodies against MLC2 and β-actin by using Western blot–ECL analysis.