Figure 4.

Effect of pre-treatment of 5-FU, oxaliplatin and irinotecan on heat-sensitivity at different temperatures, HT29 (A) and HCT116 (B) cells were pretreated with a drug for 24 h before heat treatment at 37° (□), 42°() and 45°C (▪) for 15 min. Drugs were removed form medium before heat treatment. Cell viability was determined by MTT assay 72 h after heat treatment. Data are shown as mean ± SD. *p < 0.05, compared to cells at 37°C by the Student's test. Cell survival for each termperature was corrected for heat cytotoxicity.