Table I.
Putative nitrated peptides from two-dimensional LC-MS/MS data of mouse brain ER microsomes
The search was performed against a composite IPI mouse database (version 3.35 containing both forward and randomized sequences) using Mascot with a 2-Da precursor ion mass tolerance. Listed sequences had greater than 95% probability as determined by the Peptide Prophet algorithm. All values in the table were taken directly from Scaffold output. All cysteine residues shown were modified via carbamidomethylation (+57.02 Da). Asterisks indicate nitration (+44.98 Da). See text for discussion about the validity of these identifications using more stringent criteria (parent ion tolerance of 5, 10, and 50 ppm) and about the combination of factors that may collectively generate isobaric interferences.
| IPI accession number(s) | Gene symbol | Putative nitrated peptide sequence | Mascot ion score | Mascot identity score (p < 0.05) | Observed m/z | Charge state | Mass error |
|---|---|---|---|---|---|---|---|
| ppm | |||||||
| IPI00757353 | spna2 | ALSSEGKPY*VTKEELYQNLTR | 49.89 | 41.62 | 824.094 | 3 | −395 |
| IPI00122048 | atp1a3 | KY*NTDCVQGLTHSK | 47.99 | 42.89 | 847.4022 | 2 | −1169 |
| IPI00338039 | tubb2a | LTTPTY*GDLNHLVSATMSGVTTCLR | 41.39 | 41.12 | 1377.617 | 2 | 327 |
| GHY*TEGAELVDSVLDVVRK | 52.87 | 42.24 | 1065.560 | 2 | −915 | ||
| IPI00473320 | actb | KDLY*ANTVLSGGTTMYPGIADR | 41.91 | 41.81 | 1194.098 | 2 | −404 |
| IPI00110753 | tuba1 | GHY*TIGKEIIDLVLDR | 54.92 | 42.62 | 629.350 | 3 | −510 |
| IPI00221402 | aldoa | Y*ASICQQNGIVPIVEPEILPDGDHDLKR | 57.57 | 40.33 | 1074.538 | 3 | 1.97 |
| IPI00554928 | nefl | Y*LKEYQDLLNVK | 46.98 | 43.06 | 784.920 | 2 | −1262 |
| IPI00331590 | mdh2 | HGVY*NPNKIFGVTTLDIVR | 41.32 | 42.12 | 1094.096 | 2 | −443 |
| LTLY*DIAHTPGVAADLSHIETR | 69.80 | 41.68 | 813.426 | 3 | 13.0 | ||
| GY*LGPEQLPDCLKGCDVVVIPAGVPR | 78.00 | 40.91 | 1427.742 | 2 | 18.3 | ||
| IPI00223377 | mbp | Y*LATASTMDHAR | 48.38 | 43.26 | 690.322 | 2 | −1435 |
| IPI00263013 | plp1 | MY*GVLPWNAFPGKVCGSNLLSICK | 45.9 | 41.42 | 1378.194 | 2 | −347 |
| IPI00663736 | syngap1 | Y*LKDAIGEFIR | 51.55 | 43.24 | 684.515 | 2 | −1235 |
| IPI00230192 | gnao1 | QY*KPVVYSNTIQSLAAIVR | 76.23 | 42.04 | 1097.138 | 2 | −872 |
| IPI00121550 | atp1b1 | Y*NPNVLPVQCTGKR | 43.81 | 42.84 | 844.930 | 2 | −1174 |
| IPI00465810 | sv2a | GGQY*FNDKFIGLR | 43.82 | 43.03 | 779.396 | 2 | −1269 |
| IPI00116498 | ywhaz | Y*LAEVAAGDDKK | 65.53 | 43.29 | 661.830 | 2 | −1502 |
| IPI00110658 | hba-a1 | TY*FPHFDVSHGSAQVK | 43.14 | 42.70 | 931.949 | 2 | −1059 |
| IPI00117978 | cox4i1 | DY*PLPDVAHVTMLSASQK | 74.73 | 42.41 | 1008.000 | 2 | −981 |
| IPI00132390 | ndufb4 | Y*KPAPLATLPSTLDPAEYDVSPETR | 40.68 | 41.12 | 926.476 | 3 | 376 |
| IPI00132874 | mgll | Y*DELAHMLKGLDMLVFAHDHVGHGQSEGER | 38.93 | 39.92 | 1145.760 | 3 | −383 |