Fig. 6.

Caspase-3 and -7 are dispensable for LPS- and Salmonella-induced cytokine secretion. A–D, macrophages from wild type, caspase-1^−/−, caspase-3^−/−, and caspase-7^−/− mice were stimulated with LPS for 3 h and then pulsed with ATP for 30 min, stimulated with LPS alone, pulsed with ATP alone, or infected with wild type S. typhimurium (Salm) or a flagellin-deficient mutant (Salm Flib/c−) for 1 h. Cell culture medium was analyzed for release of IL-1β (A and C), IL-18 (B), or tumor necrosis factor-α (TNF-α) (D) by ELISA. CASP1, caspase-1; CASP3, caspase-3; CASP7, caspase-7; CTRL, control; WT, wild type. Results represent mean ± standard deviation of triplicates and are representative of these three independent experiments.