. 2008 Dec;7(12):2350–2363. doi: 10.1074/mcp.M800132-MCP200

Table I.

Overview of targets and cleavage sites identified in a proteome-wide COFRADIC analysis with caspase-1

The complete list of internally located, trideuteroacetylated (AcD₃) peptides with an Asp residue in P₁ that were uniquely identified in the digested proteome of recombinant caspase-1-treated mouse Mf414 lysates is shown. Protein names are identified by using the Swiss Institute of Bioinformatics BLAST network service, and the accession numbers are according to the UniProtKB/Swiss-Prot database. Cleavage sites represent the amino acids preceding the identified peptide (i.e. P₄–P₁) as well as the P_1′. The targets are divided into two groups based on the presence of an aliphatic (cluster I) or acidic (cluster II) residue at P₄. The identified peptide sequences and modified sequences are presented. The predicted molecular mass of the full-length targets is indicated in integers. Mox, oxidized methionine; Dam, deamidation.

Accession no.	Protein name	Molecular mass	Sequence	Modified sequence	Cleavage site
		kDa
Cluster I
P97864	Caspase-7	34	`SGPINDIDANPR`	`AcD₃-SGPINDIDANPR-COOH`	`IQAD^198↓S¹⁹⁹`
P60710	β-Actin	42	`NGSGMCKAGFAGDDAPR`	`AcD₃-NDamGSGMMoxCKAGFAGDDAPR-COOH`	`LVVD^11↓N₂`
P63260	γ-Actin				`LVID^11↓N₂`
P63038	Hsp60	61	`AVAVTMGPKGR`	`AcD₃-AVAVTMMoxGPKGR-COOH`	`LLAD^49↓A⁵⁰`
			`VANNTNEEAGDGTTTATVLAR`	`AcD₃-VANNTNEEAGDGTTTATVLAR-COOH`	`LVQD^100↓V¹⁰¹`
Q8R5A3	PREL-1	74	`LVADISEAEQR`	`AcD₃-LVADISEAEQR-COOH`	`LMAD^70↓L⁷¹`
Q99KY4	Cyclin G-associated kinase	144	`GDGSEVSDEEEASFPSEER`	`AcD₃-GDGSEVSDEEEASFPSEER-COOH`	`LIAD^820↓G⁸²¹`
Q62318	TIF1b	89	`GADSTGVVAKLSPANQR`	`AcD₃-GADSTGVVAKLSPANQR-COOH`	`LSLD^684↓G⁶⁸⁵`
Q5RJH6	SMG7	127	`GSPGLKSVLSTGR`	`AcD₃-GSPGLKSVLSTGR-COOH`	`LATD^517↓G⁵¹⁸`
Q7TMY8	E3-histone	483	`EAPSNLSQASTLQANR`	`AcD₃-EAPSNLSQASTLQANR-COOH`	`VLMD^2384↓E²³⁸⁵`
O35601	FYN-binding protein	90	`GTGNLEEEQESEGETYEDIDSSKER`	`AcD₃-GTGNLEEEQDamESEGETYEDIDSSKER-COOH`	`MHSD^434↓G⁴³⁵`
P25206	MCM3	92	`DPDFTQDDQQDTR`	`AcD₃-DPDFTQDDQQDTR-COOH`	`LATD^530↓D⁵³¹`
Cluster II
P25206	MCM3	92	`FTQDDQQDTR`	`AcD₃-FTQDDQQDTR-COOH`	`DDPD^533↓F⁵³⁴`
P08113	Endoplasmin	92	`VDGTVEEDLGKSR`	`AcD₃-VDGTVEEDLGKSR-COOH`	`DEVD^26↓V²⁷`
Q91WR3	Assc2	86	`GNQVGANDADSDDELISR`	`AcD₃-GNQVGANDADSDDELISR-COOH`	`DTYD^614↓G⁶¹⁵`
Q9JLQ2	GIT2	79	`YDSVASDEDTDVETR`	`AcD₃-YDSVASDEDTDVETR-COOH`	`DQPD^390↓Y³⁹¹`
Q9WUK2	eIF-4H	27	`SLKEALTYDGALLGDR`	`AcD₃-SLKEALTYDGALLGDR-COOH`	`DEVD^92↓S⁹³`
P47713	cPLA2	85	`AAVADPDEFER`	`AcD₃-AAVADPDEFER-COOH`	`DELD^521↓A⁵²²`
Q61211	Ligatin	63	`GKPLQEQMDDLLLR`	`AcD₃-GKPLQEQMMoxDDLLLR-COOH`	`DSLD^243↓G²⁴⁴`
O88746	Target of Myb protein 1	54	`MGPDPAATNNLSSQLAGMNLGSR`	`AcD₃-MMoxGPDPAATNNDamLSSQLAGMMoxNLGSR-COOH`	`DLID^324↓M³²⁵`
Q62481	Vps72	41	`SDFDIDEGDEPSSDGEAEEPR`	`AcD₃-SDFDIDEGDEPSSDGEAEEPR-COOH`	`DEVD^58↓S⁵⁹`
Q8K2H1	Periphilin-1	44	`GTELYEDSQLSNR`	`AcD₃-GTELYEDSQLSNR-COOH`	`NTVD^291↓G²⁹²`
P60710	β-Actin	42	`GQVITIGNER`	`AcD₃-GQVITIGNER-COOH`	`ELPD^244↓G²⁴⁵`
P63260	γ-Actin
Q61152	FLP-1	50	`GAQTGGLGFNLR`	`AcD₃-GAQTGGLGFNLR-COOH`	`EVTD^424↓G⁴²⁵`