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. Author manuscript; available in PMC: 2008 Dec 5.
Published in final edited form as: Mol Pharm. 2008 Jun 17;5(5):787–794. doi: 10.1021/mp8000205

Figure 4.

Figure 4

Cellular fluorescence of NBD-C6 ceramide and its metabolites to indicate the efficiency of the Golgi apparatus to PM trafficking. Cells were incubated with 2.5 μM NBD-C6 ceramide for 30 min at 4 °C and chased at 37 °C in fresh media. The images shown represent cells at 6 h postchase. HeLa cells have significant staining of their PM indicating normal/efficient Golgi to plasma membrane transport. Leukemic cell lines HL-60/ADR HL-60/S, K562/ADR, K562/S and CEM have predominantly intracellular staining with undetectable PM staining, which is consistent with an inefficient Golgi to PM trafficking pathway. Leukemic cell outlines are shown with dashed lines to show where the plasma membrane is located.