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Dependence of CBS expression and activity on heme concentration. Western blot of yeast strain WY254 bearing plasmid expressing human CBS. Cell lysates (20 μg/lane) were electrophoresed on a 9% SDS-gel. Following Western transfer, CBS was detected using anti-CBS monoclonal antibody (upper panel). The cell lysates were assayed for CBS activity and the results were plotted (bottom panel). The strains WY61 and WY79 served as positive and negative controls, respectively.
