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Impact of chemical chaperone TMAO on human CBS expression and activity in the absence of heme. Western blot of yeast bearing plasmid expressing human CBS. Crude extracts (35 μg/lane) were electrophoresed on a 9% SDS gel. Following Western transfer, CBS was detected using anti-CBS monoclonal antibody (upper panel). Graph of corresponding CBS activities (bottom panel). Strains WY61 and WY79 served as positive and negative controls, respectively.
