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Characteristics of the various purified CBS enzymes
| Sample | Specific activity | AdoMet response | PLP response |
|---|---|---|---|
| units/mg protein | |||
| Aerobic WT hCBS fusion | 36 | YES (2.0x) | NO (1.1x) |
| Aerobic WT hCBS cleaved fusion | 30 | YES (1.7x) | NO (1.1x) |
| Anaerobic hemeless hCBS fusion | 1 | YES (2.1x) | YES (1.5x) |
| Anaerobic Fe(III) hCBS cleaved fusion | 10.5 | YES (2.3x) | NO (1.2x) |
| Anaerobic Mn(III) hCBS cleaved fusion | 3.0 | YES (3.5x) | NO (1.2x) |
| Anaerobic Co(III) hCBS cleaved fusion | 6.0 | YES (2.6x) | NO (1.1x) |
