FIGURE 1.

CA feeding increases p300 interaction with FXR in mouse liver. A, mice were tail vein-injected with Ad-3Flag-FXR or control Ad-empty and 5 days later, were fed normal (-) or 0.5% CA-supplemented (+) chow for 6 h. p300 was immunoprecipitated from liver nuclear extracts, and Flag-FXR in the immunoprecipitates was detected by Western blotting. B and C, uninfected mice were fed normal or 0.5% CA chow for 6 h, and livers were collected for further studies. B, the Shp and Cyp7a1 mRNA levels were determined by q-RTPCR, and normalized to those of 36B4. The S.E. was calculated using the Student's t test (n = 3); ^*, p < 0.05. C, interaction between endogenous p300, and FXR was detected by coimmunoprecipitation. FXR was immunoprecipitated from liver nuclear extracts, and p300 in the immunoprecipitates were detected by Western blotting. p300 and IgG heavy chain are indicated by an arrow and asterisk, respectively.