FIGURE 3.

CA feeding increases association of p300, FXR, and acetylated histone H3 K9/K14 with the Shp promoter. A and B, mice were fed normal or CA chow for 6 h, and livers were collected for ChIP analyses. B, the FXR band intensity was measured by densitometry, and the band intensity from control samples was set as 1. The S.E. was calculated using the Student's t test (n = 3). C, mice were fed CA chow for indicated times, and livers were collected for ChIP assays. D, FXR knockout (k/o) mice or normal mice were fed normal or CA chow for 6 h and association of indicated factors or acetylated histones with the Shp promoter were determined by ChIP analyses. For ChIP assays, sheared chromatin was precipitated with the indicated antibodies, and SHP promoter DNA was amplified by PCR. DNA from samples before immunoprecipitation was used for amplification in the total sample.