FXR agonists increase association of p300, FXR, and acetylated histones
at the SHP promoter in HepG2 cells. A, HepG2 cells were treated
for 6 h with FXR ligands as indicated and acetylated histone H3 K9/14 levels
at the SHP promoter. GS denotes gugglesterone. B-E, HepG2 cells were
treated with GW4064 (B) or CDCA (C) for 3 h. HepG2 cells
were treated with CDCA for indicated times (D). A-D, samples
were analyzed by ChIP assay as described in the legend to
Fig. 3. E, in re-ChIP
assays, chromatin was first immunoprecipitated with antisera to FXR and then,
re-immunoprecipitated with IgG or p300 antibody.