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Ara C blocks PKR-dependent phosphorylation in ΔE3L mutant virus-infected cells. Whole cell extracts were prepared from uninfected (–) or ΔE3L virus-infected (+) cells at 10 h after infection, either untreated (–) or treated (+) with Ara C, and analyzed by Western immunoblot assay with antibody against IRF-3. α-Tubulin was used as a loading control. Cells used were as follows: PKR-sufficient parental (PKR+) and knockdown control (PKRkd-con); PKR-deficient knockdown (PKRkd).
