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. 2008 Dec 12;283(50):35106–35117. doi: 10.1074/jbc.M806874200

FIGURE 3.

FIGURE 3.

Effect of CHOP on ASNS-driven transcription activated by HisOH or ATF4. A diagram showing the structure of the ASNS-173/+51 promoter/luciferase reporter (A). HepG2 cells were co-transfected with 0.5 μg/well of the ASNS-173/+51 promoter/luciferase reporter plasmid and the CHOP-pcDNA3.1 expression plasmid at the amounts indicated. To activate ATF4-dependent transcription, half of the cells were treated with 2 mm HisOH for 12 h (B) and the other half were co-transfected with 10 ng/well of ATF4-pcDNA3.1 expression plasmid (C). The total amount of transfected DNA was kept constant among experimental groups by the addition of pcDNA3.1 plasmid. Cell extracts were assayed for luciferase activity as described under “Materials and Methods.” Each experiment was repeated with three different batches of cells, and the results shown represent the means ± S.E.