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. 2008 Dec 12;283(50):34855–34863. doi: 10.1074/jbc.M805844200

FIGURE 2.

FIGURE 2.

Analysis of CFHL1 and CFH binding to BbCRASP-2 mutants. Purified GST-BbCRASP-2 (positive control), GST-BbCRASP-2 mutants as well as purified GST protein (negative control) were immobilized onto microtiter plates and incubated with purified CFH or CFHL1 as described under “Experimental Procedures.” Binding of CFH or CFHL1 was then assessed by an ELISA together with a polyclonal anti-CFH antibody. A, BbCRASP-2 mutants representing binding region 2, B, BbCRASP-2 mutants representing binding region 3; C, BbCRASP-2 mutants representing binding region 4. Experiments were performed at least three times, and one representative experiment is shown. Bars represent the mean of triplicates within one experiment ± S.D. One-way analysis of variance was performed to analyze statistical significance in CFH/CFHL1 binding between BbCRASP-2 and mutant proteins, and significant differences from the wild-type (p < 0.001) are indicated by asterisks.