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A, spectral titration of ΔG810 mutant FMN/heme bidomain. B, absorbance versus potential plots of ΔG810 mutant heme/FMN bidomain at 478 nm, 501 nm (FMN maximum), 553 nm, and 650 nm (heme components show maximal change) are fit to the three-electron modified Nernst equation (Equation 2) as given under “Experimental Procedures.” The weight of components during fitting was changed depending on the wavelengths used for obtaining the fit.
