TABLE 1.
The redox and structural properties of some FMN-containing enzymes
| Enzyme | Em | E ox/sq | E sq/hq | Observable semiquinone | Protein reference | H-bonding to FMN N5 | PDB entry code | Structure reference |
|---|---|---|---|---|---|---|---|---|
| mV | mV | mV | ||||||
| A. nidulans flavodoxin | –334 | –221 | –447 | Blue | (63) | -NH- (ox) | 1CZN | (22) |
| -C=O (sq,hq) | 1CZL | |||||||
| C. beijerinckii flavodoxin | –245 | –92 | –399 | Blue | (64) | -NH- (ox) | 5NLL | (23) |
| -C=O (sq,hq) | 2FOX | |||||||
| D. vulgaris flavodoxin | –293 | –149 | –438 | Blue | (65) | -NH- (ox) | 3FX2 | (24) |
| -C=O (sq, hq) | 4FX2 | |||||||
| Rabbit cytochrome P450 reductase | –190 | –110 | –270 | Blue | (33) | -C=O | 1AMO | (62) |
| Rat nNOS FMN domain | –246 | –179 | –314 | Blue | (32) | -C=O | 1TLL | (54) |
| Rat nNOS ΔG810 FMN domain | –235 | –280 | –190 | Red | This work | -NH- expected | N/Aa | N/A |
| B. megaterium P450 BM3 FMN domain | –200 | –240 | –160 | Red | (20) | -NH- | 1BVY | (18) |
| Free FMN | –219 | –314 | –124 | (7) |
a
N/A, not applicable