TABLE 2.

Reduction of FMN by dithionite in the FMN domain of nNOS ΔG810 mutant Each of the two phases, sq formation and its further reduction, at 391 nm, was fit with a single exponential equation while the curves at 468 nm were fit with a double exponential equation except that at pH 9.0. The rate constants are listed as mean ± S.D. of at least five individual measurements.

Wavelength	pH	k (formation of sq)	k (reduction to hq)
nm		s–1
391	7.0	0.872 ± 0.038	0.0322 ± 0.0010
	8.0	0.449 ± 0.051	0.0165 ± 0.0008
	9.0	0.412 ± 0.017
		k (fast)	k (slow)
468	7.0	0.481 ± 0.054	0.0541 ± 0.0041
	8.0	0.422 ± 0.061	0.0324 ± 0.0110
	9.0	0.388 ± 0.010