TABLE 3.

Steady-state enzymatic activity assays for the full-length wild-type nNOS and the ΔG810 mutant Buffer conditions for each assay are described under “Experimental Procedures.” The turnover numbers are the mean with standard deviation for at least three measurements each. The + or – signs represent whether the specified buffer component is present or absent, respectively.

Assay	Enzyme	CaM CaCl2	CAT SOD	l-Arg	Turnover
					min–1
NO synthesis
	Wild type	+	+	+	74 ± 8
		+	–	+	55 ± 5
		–	+	+	N/Da
	Mutant	+	+	+	3.3 ± 0.7
		+	–	+	2.0 ± 0.2
		–	+	+	N/D
NADPH oxidation
	Wild type	+	+	+	140 ± 9
		+	–	+	154 ± 10
		+	+	–	336 ± 6
		–	+	+	13 ± 5
	Mutant	+	+	+	17 ± 2
		+	–	+	19 ± 1
		+	+	–	19 ± 3
		–	+	+	14 ± 4
Cyt. c reduction
	Wild type	+	–	N/Ab	4270 ± 180
		–	–	N/A	670 ± 31
	Mutant	+	–	N/A	4400 ± 183
		–	–	N/A	4490 ± 135

^aN/D, not detectable

^bN/A, not applicable