FIGURE 3.

Inhibition the Aβ internalization by RAP in TR-BBB cells. A, the uptake assay of ¹²⁵I-Aβ or ¹²⁵I-Aβ·apoE complex in the absence or presence of 500 nm RAP. The mean ± S.E. in four independent assays is shown. **, p < 0.01; ANOVA. B, the internalized and cell-bound ¹²⁵I-Aβ in TR-BBB cells were measured in the absence or presence of 500 nm RAP and 3μm unlabeled Aβ-(1–40). The mean ± S.E. in six independent assays is shown. **, p < 0.01; ANOVA. B, the uptake assay of ¹²⁵I-Aβ was performed with 0, 10, 30, 62.5, 125, 300, 500, and 1000 nm RAP in the uptake medium. The mean ± S.E. in 3–7 independent assays is shown. D, the uptake assays in the presence of 3 μm unlabeled Aβ-(1–40), 10 μg/ml anti-RAGE antibody, 10 μg/ml control IgG, or 100 μm verapamil. The mean ± S.E. in four independent assays is shown.