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. 2008 Dec 12;283(50):34554–34562. doi: 10.1074/jbc.M801487200

FIGURE 6.

FIGURE 6.

Lack of Aβ internalization in fibroblasts endogenously expressing LRP1. A, immunoblot analysis of TR-BBB, MEF-1 (wild-type), PEA10 (LRP1 heterozygous-deficient), and PEA13 (LRP1 homozygous-deficient) cells using a polyclonal anti-LRP1 antibody R488. B, Aβ uptake assay in MEF cells. TR-BBB (filled squares), MEF-1 (open squares), and PEA13 (open circles) cells were incubated with 125I-Aβ for 0, 10, 30, 60, and 120 min. The mean ± S.E. in three independent assays is shown. C, the uptake assay of tPA in TR-BBB (filled squares), MEF-1 (open squares), PEA10 (open triangles), and PEA13 (open circles at 37 °C, crosses at 4 °C) cells for 0, 10, 30, and 60 min (MEF1, PEA10, and PEA13 cells) or for 0, 15, 30, and 45 min (TR-BBB cells). The mean ± S.E. in three independent assays is shown.