FIGURE 2.

R6G transport is drastically diminished in S558Y. The transport activity of WT and mutant Pdr5s was estimated with a flow cytometry-based efflux assay with R6G as the fluorescent substrate. The null strain (Δpdr5 = AD1–7) and yeast strains containing the WT (JG2000) and mutant (JG2009) Pdr5 were used in these experiments. The assay was carried out as described previously (21), and the representative histograms from four independent experiments are depicted. The strains used are labeled on the figure.