FIGURE 4.

Pdr5-mediated ATP hydrolysis. A, Vi-sensitive ATPase activity of double-copy WT (•) or S558Y (▪) Pdr5 in purified PM vesicles (10 mg per assay) was assayed as described previously (5) in the presence of increasing concentrations of ATP (0–3 mm ATP). B, clotrimazole sensitivity of ATPase activity was monitored in WT purified plasma membrane vesicles in the absence (•) or presence (▪) of 25 μm clotrimazole. C, clotrimazole sensitivity of ATPase activity was monitored in the Pdr5 mutant, S558Y, purified plasma membrane vesicles in the absence (•) or presence (▪) of 25 μm clotrimazole. Representative plots are shown (n = 3).