FIGURE 9.
Drug sensitivity of WT Pdr5 and S558Y and N242K. A, 10-fold serial dilutions (0.5 × 106 cells to 0.5 × 103 cells in 5 μl of sterile H2O) were plated on YPD and on YPD containing 3.1 μm cycloheximide or 22.5 μm clotrimazole. Plates were scanned after 96 h of incubation at 30 °C. Row 1, R-1 (Δpdr5) cells; row 2, WT (R-1 + pSS607) cells; row 3, R-1 + pS558Y; rows 4 and 5, two independent transformants of R-1 + pN242K. B, quantitative growth analysis of WT (•), S558Y (▴), and N242K (▪) in the presence of increasing concentrations of cyh. Growth in the absence of cyh was taken to be 100% in each curve. C, growth of WT (•), S558Y (▪), and N242K (▴) was compared as described in B, except that the experiment was carried out in the presence of increasing concentrations of clotrimazole.