TABLE 1.
Yeast strains used in this study All the strains are isogenic derivatives of AD1–7, with the exception of JG365-3B.
| Strain | Relevant genotype | Source or Ref. |
|---|---|---|
| JG365-3B | Mat a PDR5 PDR1-3 ura3 leu2 | 35 |
| AD1–7 | MATα PDR1-3 ura3 his1 yor1 pdr5 snq2 pdr10 ycf1 pdr11 pdr3 | 36; ura3 derivative made by J. Golin |
| JG2000 | AD1–7 + pSS607 | 5 |
| JG2001 | ura3, PDR5 5-FOA derivative of JG2000 | 5 |
| JG2003 | JG2001 + pS558Y | This study |
| JG2004 | JG2001 + pSS607 | This study |
| JG2005 | AD1–7 + pS558Y | This study |
| JG2009 | ura3, S558Y derivative of JG2003 | This study |
| JG2010 | JG2009 + pS558Y | This study |
| R-1 | Δpdr5::KANMX4 replaces PDR5 in JG2001 | This study |
| JG2011 | R-1 + pS558Y | This study |
| JG2012 | R-1 + pS558A | This study |
| JG2015 | R-1 + pSS607 (wild-type control) | This study |
| JG2016 | R-1 + pN242K | This study |
| JG2020 | clo-3-0 + pS558Y, N242K | This study |
| JG2021 | R-1 + pS558Y, N242K | This study |